Journal: Investigative Ophthalmology & Visual Science

Article Title: Neutrophil and T-Cell Homeostasis in the Closed Eye

doi: 10.1167/iovs.17-22449

Figure Lengend Snippet: Analysis of the T-cell populations from the closed eye following 1 vs. 7 hours of sleep. (A) Total number of CD3 + T cells, CD4 + helper T cells, CD8 + cytotoxic T cells, and Th17 cells measured by flow cytometry. (B) Relative percentage of all CD4 + T-helper cells out of all CD3 + T cells. (C) Relative percentage of all Th17 cells out of all CD4 + T cells. *P < 0.05, n = 9.

Article Snippet: Interdaily variations in flow cytometry acquisition were controlled for using the Application Settings feature in BD FACS Diva software.

Techniques: Flow Cytometry

Journal: BMC Bioinformatics

Article Title: A methodology for exploring biomarker – phenotype associations: application to flow cytometry data and systemic sclerosis clinical manifestations

doi: 10.1186/s12859-015-0722-x

Figure Lengend Snippet: Example random walk. Gene Set Enrichment Analysis random walk for a flow cytometry set comprised of 27 variables

Article Snippet: The methods utilized in the work involve: (1) clinical and peripheral blood flow cytometry data ( I mmune R esponse I n S cleroderma, IRIS) from consented patients followed at the Johns Hopkins Scleroderma Center. (2) machine learning (Conditional Random Forests - CRF) coupled with Gene Set Enrichment Analysis (GSEA) to identify subsets of FC variables that are highly effective in classifying ILD patients; and (3) stochastic simulation to design, train and validate ILD risk screening tools.

Techniques: Flow Cytometry

Journal: Frontiers in Immunology

Article Title: Harmonization and qualification of intracellular cytokine staining to measure influenza-specific CD4 + T cell immunity within the FLUCOP consortium

doi: 10.3389/fimmu.2022.982887

Figure Lengend Snippet: Parameters selected for the ICS consensus assay.

Article Snippet: Eight laboratories participated in pilot study 2 and provided data of their analysis of the flow cytometry data for IFNγ and polypositive CD4 + and CD8 + T cells ( ).

Techniques: Cell Counting, Staining

Journal: Frontiers in Immunology

Article Title: Harmonization and qualification of intracellular cytokine staining to measure influenza-specific CD4 + T cell immunity within the FLUCOP consortium

doi: 10.3389/fimmu.2022.982887

Figure Lengend Snippet: Pilot Study 2: Variability of post-analytical analysis between laboratories. Eight laboratories participated in pilot study 2. Each laboratory analyzed fcs files from 10 unstimulated (background) and 10 stimulated samples (x-axis). Each dot represents the number of Ag-specific live IFNγ or polypositive CD4 + or CD8 + cells per million parent cells from one laboratory. The horizontal line with error bars indicate the mean +/- standard deviation per sample. IFN, interferon; PP, polypositive.

Article Snippet: Eight laboratories participated in pilot study 2 and provided data of their analysis of the flow cytometry data for IFNγ and polypositive CD4 + and CD8 + T cells ( ).

Techniques: Standard Deviation

Journal: Frontiers in Immunology

Article Title: Harmonization and qualification of intracellular cytokine staining to measure influenza-specific CD4 + T cell immunity within the FLUCOP consortium

doi: 10.3389/fimmu.2022.982887

Figure Lengend Snippet: Pilot Study 2: Gating practices for cytokine secreting CD4 + T cells from each of the participating laboratories, and examples of a single cytokine gate (IFNγ) from each of the laboratories Eight laboratories participated in pilot study 2-, gating strategy did not show single IFNγ-secreting cells; IFN, interferon.

Article Snippet: Eight laboratories participated in pilot study 2 and provided data of their analysis of the flow cytometry data for IFNγ and polypositive CD4 + and CD8 + T cells ( ).

Techniques: